1. UDP-glucose:3-deoxyanthocyanidin 5-O-glucosyltransferase from Sinningia cardinalis. Nakatsuka, T.and Nishihara, M. Planta 232:383-392. 2010. [PubMed].
  2. Strict de novo methylation of the 35S enhancer sequence in gentian. Mishiba, K., Yamasaki, S., Nakatsuka, T., Abe, Y.,Daimon, H., Oda, M. and Nishihara, M. PLoS One 5(3): e9670. 2010. [PubMed]
  3. Two R2R3-MYB genes, homologues of petunia AN2, regulate anthocyanin biosyntheses in flower tepals, tepal spots and leaves of asiatic hybrid lily. Yamagishi, M., Shimoyamada, Y., Nakatsuka, T., Masuda, K. Plant and Cell Physiology: 51: 463-474. 2010. [PubMed]
  4. Genetic engineering of novel flower colour by suppression of anthocyanin modification genes in gentian. Nakatsuka, T., Mishiba, K., Kubota, A. Abe, Y., Yamamura, Y., Nakamura, N., Tanaka, Y. and Nishihara, M. Journal of Plant Physiol 167: 231-237. 2010. [PubMed]
  5. Genetic engineering of novel flower colors in floricultural plants: Recent advances via transgenic approaches. Nishihara, M. and Nakatsuka, T. Methods in Molecular Biology 589: Protocols for in vitro propagation of ornamental plants. S. Mohan Jain and Sergio J. Ochatt (eds). The Humana Press Inc. pp. 325-347. 2010. [PubMed]
  6. A novel factor FLOURY ENDOSPERM2 is involved in regulation of rice grain size and starch quality. She, K.C., Kusano, H., Koizumi, K., Yamakawa, H., Hakata, M., Imamura, T., Fukuda, M., Naito, N., Tsurumaki, Y., Yaeshima, M., Tsuge, T., Matsumoto, K., Kudoh, M., Itoh, E., Kikuchi, S., Kishimoto, N., Yazaki, J., Ando, T., Yano, M., Aoyama, T., Sasaki, T., Satoh, H., and Shimada, H. Plant Cell 22: 3280-3294. 2010. [PubMed]
  7. Acquired resistance to the rice blast in transgenic rice accumulating the antimicrobial peptide thanatin. Imamura, T., Yasuda, M., Kusano, H., Nakashita, H., Ohno, Y., Kamakura, T., Taguchi, S., and Shimada, H. Transgenic Research 19: 15-424. 2010. [PubMed]
  8. Metabolome and photochemical analysis of rice plants overexpressing Arabidopsis NAD kinase gene. Takahara, K., Kasajima, I., Takahashi, H., Hashida, S.N., Itami, T., Onodera, H., Toki, S., Yanagisawa, S., Kawai-Yamada, M., and Uchimiya, H. Plant Physiology 152: 1863-1873. 2010. [PubMed]
  9. Principal component and hierarchical clustering analysis of metabolites in destructive weeds; Polygonaceous plants. Miyagi, A., Takahashi, H., Takahara, K., Hirabayashi, T., Nishimura, Y., Tezuka, T., Kawai-Yamada, M., and Uchimiya, H. Metabolomics 6, 146-155. 2010.[Link]
  10. Nicotinate/nicotinamide mononucleotide adenyltransferase-mediated regulation of NAD biosynthesis protects guard cells from reactive oxygen species in ABA-mediated stomatal movement in Arabidopsis. Hashida, S.N., Itami, T., Takahashi, H., Takahara, K., Nagano, M., Kawai-Yamada, M., Shoji, K., Goto, F., Yoshihara, T., and Uchimiya, H. Journal of Experimental Botany 61: 3813-3825. 2010. [PubMed]
  11. Characterization of a cellobiohydrolase (MoCel6A) produced by Magnaporthe oryzae. Takahashi, M., Takahashi, H., Nakano, Y., Konishi, T., Terauchi, R., and Takeda, T. Appl. Environ. Microbiol. 76: 6583-6590. 2010. [PubMed]
  12. Genetic relationships in the genus Gentiana based on chloroplast DNA sequence data and nuclear DNA content. Mishiba, K., Yamane, K., Nakatsuka, T., Nakano, Y., Yamamura, S., Abe, J., Kawamura, K., Takahata, Y. and Nishihara, M. Breeding Science 59: 119-127. 2009. [J-STAGE]
  13. Over-expression of Arabidopsis FT gene reduces juvenile phase and induces early fowering in ornamental gentian plants. Nakatsuka, T., Abe, Y., Kakizaki, Y., Kubota, A., Shimada, N. and Nishihara, M. Euphytica 168: 113-119. 2009. [SpringerLink]
  14. Metabolite profiling by capillary electrophoresis mass spectrometry reveals aberrant putrescine accumulation associated with idiopathic symptoms of gentian plants. Takahashi, H., Munemura, I., Nakatsuka, T., Nishihara, M. and Uchimiya, H. 2009. The Journal of Horticultural Science and Biotechnology 84: 312-318. 2009. [LINK]
  15. Development of codominant DNA marker distinguishing pink from blue flowers in Gentiana scabra. Kakizaki, Y., Nakatsuka, T., Kawamura, H., Abe, J., Abe, Y., Yamamura S. and Nishihara, M. Breeding Research 11: 9-14. 2009. (in Japanese with English abstract) [J-STAGE]
  16. Identification of gentian cultivars using SCAR markers based on intron-length polymorphisms of flavonoid biosynthetic genes. Shimada, N., Nakatsuka, T., Nakano, Y., Kakizaki, Y., Abe, Y., Hikage, T. and Nishihara, M. Scientia Horticulturae 119: 292-296. 2009. [ScienceDirect]
  17. Pleiotropic modulation of carbon and nitrogen metabolism in Arabidopsis plants overexpressing NAD kinase2 gene. Takahashi, H., Takahara, K., Hashida, S.N., Fujimori, T., Kawai-Yamada, M., Yamaya, T., Yanagisawa, S., and Uchimiya, H. Plant Physiol. 151, 100-113. 2009. [PubMed]
  18. Metabolite profiling by capillary electrophoresis mass spectrometry reveals aberrant putrescine accumulation associated with idiopathic symptoms of gentian plants. Takahashi H, Munemura I, Nakatsuka T, Nishihara M, and Uchimiya H.J. Hortic. Sci. Biotechnol. 84, 113-119. 2009. [Link]
  19. Polyamines as a common source of hydrogen peroxide in host- and nonhost-hypersensitive response during pathogen infection. Yoda H, Fujimura K, Takahashi H, Munemura I, Uchimiya H, and Sano H.Plant Mol. Biol. 70, 103-112. 2009. [PubMed]
  20. The role of NAD biosynthesis in plant development and stress responses. Hashida SN, Takahashi H, and Uchimiya H. Ann. Bot. 103, 819-824. 2009. [PubMed]
  21. Functional genomics of the Dof transcription factor family genes in suspension-cultured cells of Arabidopsis thaliana. Tsujimoto-Inui Y, Naito Y, Sakurai N, Suzuki H, Sasaki R, Takahashi H, Ohtsuki N, Nakano T, Yanagisawa S, Shibata D, Uchimiya H, Shinshi H, and Suzuki K. Plant Biotechnol. 26: 15-18. 2009. [Link]
  22. Identification and characterization of R2R3-MYB and bHLH transcription factors regulating anthocyanin biosynthesis in gentian flowers. Nakatsuka, T., Haruta, K.S., Pitaksutheepong, C., Abe, Y., Kakizaki, Y., Yamamoto, K., Shimada, N., Yamamura, S. and Nishihara, M. Plant and Cell Physiology 49: 1818-1829. 2008. [PubMed]
  23. Cloning and characterization of the UDP-glucose:anthocyanin 5-O-glucosyltransferase gene from blue-flowered gentian. Nakatsuka, T., Sato, K., Takahashi, H., Yamamura, S., and Nishihara, M. Journal of Experimental Botany 59: 1241-1252. 2008. [PubMed]
  24. RIP and RALyase cleave the sarcin/ricin domain, a critical domain for ribosome function, during senescence of wheat coleoptiles. Sawasaki, T., Nishihara, M. and Endo, Y. Biochemical and Biophysical Research Communications 370: 561-565. 2008. [SpringerLink]
  25. Flower color modification of gentian plants by RNAi-mediated gene silencing. Nakatsuka, T., Mishiba, K., Abe, Y., Kubota, A., Kakizaki, Y., Yamamura, S. and Nishihara, M. Plant Biotechnology 25: 61-68. 2008. [J-STAGE]
  26. FioreDB: a database of phenotypic information induced by the chimeric repressor silencing technology (CRES-T) in Arabidopsis and floricultural plants. Mitsuda, N., Umemura, Y. Ikeda, M., Shikata, M., Koyama, T., Matsui,K., Narumi, T., Aida, R., Sasaki, K., Hiyama, T., Higuchi, T., Ono, M., Isuzugawa, K., Saitoh, K., Endo,R., Ikeda, K., Nakatsuka, T., Nishihara, M., Yamamura, S., Yamamura, T., Terakawa, T., Ohtsubo, N., and Ohme-Takagi, M. Plant Biotechnology 25: 37-44. 2008. [J-STAGE]
  27. Gentians: From gene cloning to molecular breeding. Nishihara, M., Nakatsuka, T., Mizutani-Fukuchi, M., Tanaka, Y., Yamamura, S. Floricultural and Ornamental Biotechnology V, published by Global Science Books, UK. pp. 57-67. 2008.
  28. The cell death factor, cell wall elicitor of rice blast fungus (Magnaporthe grisea) causes metabolic alterations including GABA shunt in rice cultured cells. Takahashi H, Matsumura H, Kawai-Yamada M, and Uchimiya H. Plant Sig. Behav. 11: 945-953. 2008. [PubMed]
  29. Difference in metabolite levels between photoatotrophic and photomixotrophic cultures of Synechocystis sp. PCC 6803 examined by capillary electrophoresis electrospray ionization mass spectrometry. Takahashi H, Uchimiya H, and Hihara Y. J. Exp. Bot. 59: 3009-3018. 2008. [PubMed]
  30. CO2 regulator SLAC1 and its homologues are essential for anion homeostasis in plant cells. Negi J, Matsuda O, Nagasawa T, Oba Y, Takahashi H, Kawai-Yamada M, Uchimiya H, Hashimoto M, and Iba K. Nature 452: 483-486. 2008. [PubMed]
  31. NMR based structure-activity relationship analysis of an antimicrobial peptide, thanatin, engineered by site-specific chemical modification: Activity improvement and spectrum alteration. Imamura T, Yamamoto N, Tamura A, Murabayashi S, Hashimoto S, Shimada H, and Taguchi S. Biochem Biophys Res Commun. 369: 609-615. 2008. [PubMed]
  32. Rice exonuclease-1 homologue, OsEXO1, that interacts with DNA polymerase lambda and RPA subunit proteins, is involved in cell proliferation. Furukawa T, Imamura T, Kitamoto HK, and Shimada H. Plant Mol Biol. 66: 519-531. 2008. [PubMed]
  33. Induction of differential flower pigmentation patterns by RNAi using promoters with distinct tissue-specific activity. Nakatsuka, T., Pitaksutheepong, C., Yamamura, S. and Nishihara, M. Plant Biotechnology Reports 1: 251-257. 2007. [SpringerLink]
  34. Pathogenesis-related protein 1 homologue is an antifungal protein in Wasabia japonica leaves and confers resistance to Botrytis cinerea in transgenic tobacco. Kiba, A., Nishihara, M., Nakatsuka , T. and Yamamura, S. Plant Biotechnology 24: 247-253. 2007. [J-STAGE]
  35. Flower color alteration in Lotus japonicus by modification of the carotenoid biosynthetic pathway.Suzuki, S., Nishihara, M,, Nakatsuka, T., Misawa, N., Ogiwara, I. and Yamamura, S. Plant Cell Reports 26: 951-959. 2007. [PubMed]
  36. Production of red-flowered plants by genetic engineering of multiple flavonoid biosynthetic genes. Nakatsuka, T., Abe, Y., Kakizaki, Y., Yamamura, S. and Nishihara, M. Plant Cell Reports 26: 1951-1959. 2007. [PubMed]
  37. Arabidopsis thaliana nicotinate/nicotinamide mononucleotide adenyltransferase (AtNMNAT) is required for pollen tube growth. Hashida SN, Takahashi H, Kawai-Yamada M, and Uchimiya H. Plant J. 49, 694-703. 2007. [PubMed]
  38. A rice dihydrosphingosine C4 hydroxylase (DSH1) gene, which is abundantly expressed in the stigmas, vascular cells and apical meristem, may be involved in fertility. Imamura T, Kusano H, Kajigaya Y, Ichikawa M, and Shimada H. Plant Cell Physiol. 48: 1108-1120. 2007. [PubMed]
  39. Two different transposable elements inserted in flavonoid 3', 5'-hydroxylase gene contribute to pink flower coloration in Gentiana scabra. Nakatsuka, T., Nishihara, M., Mishiba, K., Hirano, H. and Yamamura, S. Molecular Genetics and Genomics 275: 231-241. 2006. [PubMed]
  40. Heterologous expression of two gentian cytochrome P450 genes can modulate the intensity of flower pigmentation in transgenic tobacco plants. Nakatsuka, T., Nishihara, M., Mishiba, K. and Yamamura, S. Molecular Breeding 17: 91-99. 2006. [SpringerLink]
  41. Dominant inheritance of white-flowered and herbicide-resistant traits in transgenic gentian plants. Nishihara, M., Nakatsuka, T., Hosokawa, K., Yokoi, T., Abe, Y., Mishiba, K. and Yamamura S. Plant Biotechnology 23: 25-32. 2006. [J-STAGE]
  42. Production of dwarf potted gentian using wild-type Agrobacterium rhizogenes. Mishiba, K., Nishihara, M., Abe, Y., Nakatsuka, T., Kawamura, H., Kodama, K., Takesawa, T., Abe, J. and Yamamura, S. Plant Biotechnology 23: 33-38. 2006. [J-STAGE]
  43. Transgenic Phalaenopsis plants with resistance to Erwinia carotovora produced by introducing wasabi defensin gene using Agrobacterium method. Sjahril, R., Chin, DP., Khan, RS., Yamamura, S., Nakamura, I., Amemiya, Y. and Mii, M. Plant Biotechnology 23: 191-194. 2006. [J-STAGE]
  44. Isolation and characterization of a cDNA encoding polyketide reductase in Lotus japonicus. Shimada, N., Nakatsuka, T., Nishihara, M., Yamamura, S., Ayabe, S. and Aoki, T. Plant Biotechnology 23: 509-513. 2006. [J-STAGE]
  45. Transgenic potatoes expressing wasabi defensin peptide confers partial resistanceto gray mold (Botrytis cinerea). Khan, RS., Nishihara, M., Yamamura, S., Nakamura, I. and Mii, M. Plant Biotechnology 23: 179-183. 2006. [J-STAGE]
  46. Bisexual sterility conferred by the differential expression of Barnase and Barstar: a simple and efficient method of transgene containment. Kobayashi, K., Munemura, I., Hinata, K. and Yamamura, S. Plant Cell Reorts 25: 1347-1354. 2006. [PubMed]
  47. Chloroplast NAD kinase is essential for energy transduction through the xanthophyll cycle in photosynthesis. Takahashi H, Watanabe A, Tanaka A, Hashida SN, Kawai-Yamada M, Sonoike K, and Uchimiya H. Plant Cell Physiol. 47: 1678-1682. 2006. [PubMed]
  48. Evaluation of metabolic alteration in transgenic rice overexpressing dihydroflavonol-4-reductase. Takahashi H, Hayashi M, Goto F, Sato S, Soga T, Nishioka T, Tomita M, Kawai-Yamada M, and Uchimiya H. Ann. Bot. 98: 819-825. 2006. [PubMed]
  49. Polyamine oxidase is one of the key elements for oxidative burst to induce programmed cell death in tobacco cultured cells. Yoda H, Hiroi Y, Sano H. Plant Physiol. 142: 193-206. 2006. [PubMed]